❯

BOOK A CALL

Book Call On Your Favorite Time

I'm a Buyer I'm a Supplier

🗘

By Signing Up. I agree to TradeKey.com Terms of Use, Privacy Policy, IPR and receive emails related to our services

RNasin

Precio FOB

Obtener el precio más reciente

- Minimum Order

País:

China

N º de Modelo:

Precio FOB:

(Negociable) Obtener el precio más reciente

Lugar de origen:

China

Precio de pedido mínimo:

Cantidad de pedido mínimo:

Detalle de embalaje:

El tiempo de entrega:

7-15 days

Capacidad de suministro:

Tipo de pago:

T/T, L/C, D/A, D/P, Western Union, Money Gram, PayPal, Other

Grupo de productos :

Molecular Biology

Contactar ahora

Lablead biotech

China

200 Puntos de confianza

1st Año

Persona de contacto Ying

Contactar ahora

Descripción del producto

RNasin CATï¼šR***0 Price: U.S.ï¼„*5.********0U;$**********0U; $**********0U (negotiable)

product ID: R***0
Storage conditions:Stored at **0â„ƒ. Avoid foaming, violent oscillation or stirring.

Product description RNasin is a recombinant protein expressed by Escherichia coli and forms a 1:1 complex with RNase A, showing high non-competitive inhibition of RNase (Ki=3×****0 M). The reaction is reversible. The complex can be dissociated by urea and basophosphoric reagents, and the RNase can be reactivated while the inhibitor is irreversibly inactivated. Unlike other competitive inhibitors (nucleic acids, inorganic phosphoric acids), this product has protein properties and can be easily removed from the reaction system by phenol treatment.

activity definition
The amount of enzyme required to inhibit *0% of the activity of 5 ng RNase A is defined as 1 unit of activity (U).
Experimental steps:
The first chain cDNA synthesis (take *0 ul reaction system as an example)
1.Add the following reagents according to the table.

Components	Volume
Total RNA/mRNA	0 ng5μg/****0 ng
Oligo(dT)*8(0.5μg /μl)or	1μl
Random Primer(0.1μg/μl) or	1μl
GSP(Gene Specific Primer)	2 pmol
dNTP Mixture (*0 mM each)	1μl
5× RT Buffer	4 μl
RNase Inhibitor(*0 units/μl)	0.5 - 1μl
m-MLVe	1μl
RNase free H2O to final volume	*0 μl

2. Gently blending
If use Oligo (dT) *8 or gene specific primers (GSP), *2â„ƒ *0 min incubation.
If use the Random Primer, *5â„ƒ incubation for *0 min, *2 â„ƒ *0 min incubation.5 min
3. *0â„ƒ heating deactivation m - MLV.
RT-PCR
Reverse transcription of cDNA can be directly used in the PCR reaction or stored in - *0â„ƒ.

País:	China
N º de Modelo:	-
Precio FOB:	(Negociable) Obtener el precio más reciente
Lugar de origen:	China
Precio de pedido mínimo:	-
Cantidad de pedido mínimo:	-
Detalle de embalaje:	-
El tiempo de entrega:	7-15 days
Capacidad de suministro:	-
Tipo de pago:	T/T, L/C, D/A, D/P, Western Union, Money Gram, PayPal, Other
Grupo de productos :	Molecular Biology