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Sticky Recombinant Streptavidin

Sticky Recombinant Streptavidin

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Minimum Order

Place of Origin:

-

Price for Minimum Order:

-

Minimum Order Quantity:

1 Piece

Packaging Detail:

bottle/tube

Delivery Time:

within 7days based on stock available

Supplying Ability:

10 Liter per Month

Payment Type:

T/T

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SHANGHAI KEKAI BIOTECHNOLOGY CO., LTD

SHANGHAI KEKAI BIOTECHNOLOGY CO., LTD

China

Miembro Básico

Persona de contacto Louis

Shanghai, Shanghai

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Description

 
 Sticky Recombinant Streptavidin
 
CAS：********1
 
Brief introduction
 
Name: Sticky Recombinant Streptavidin (S-rSA)
 
Specification: 1 mg, *0 mg, **0 mg, **0 mg
 
Product form: lyophilized powder, the solution before lyophilization is 5mM PB (4M Na2HPO4, 1mM NaH2PO4, pH7.4) solution
 
Molecular weight: subunit monomer *3.5kDa, tetramer *4kDa
 
Isoelectric point: 6.*4
 
Theoretical activity: *8.1 U/mg protein (S-rSA: Biotin=1:4 (mol: mol))
 
Activity:≥ *5 U/mg protein (determined by modified Green method)
 
Purity:≥ *5% (SDS-PAGE detection)
 
Concentration determination: **0nm absorption method, molar absorption coefficient 1.*8 × **5 (mol/L) - 1 • cm*1, that is, c (g/L)=A**0nm/2.*0
 
Endotoxin:≤ 5EU/mg
 
Source: Escherichia coli
 
Storage condition: - *0℃
 
Validity: 2 years
 
Streptavidin (SA) is a homotetramer protein secreted by Streptomyces avidinii during its growth. Like avidin, one mole of streptavidin can bind four moles of biotin, which has high affinity with biotin. Because SA does not contain sugar groups, it has a lower non-specific binding level than avidin in detection and application, and has been widely used in enzyme-linked immunosorbent assay, immunohistochemistry, time-resolved immunofluorescence (TRFIA), quantitative PCR, single-stranded DNA preparation, biological component purification, monoclonal antibody preparation and other biotechnology fields.
 
However, the adsorbability of streptavidin is poor, and it is difficult to adsorb onto NC membrane or embed it in practical applications. Sticky recombinant streptavidin (S-rSA) is mainly used to solve this problem. It not only has the core sequence related to activity, but also has better adsorbability, stability, solubility and other aspects than natural SA, especially on NC membrane.
 
Scope of application
 
In many applications, such as immunocytochemistry, ELISA and immunoblotting, streptavidin alkaline phosphatase can be used to detect biotinylated molecules, such as antibodies.
 
Immunocytochemistry
 
For most applications, 1:**0 working dilution in TBS is sufficient. At this dilution, about ***0 tests (**0ul per slide) can be carried out for each package. It is recommended to use TBS instead of PBS in all diluents and washings to maintain maximum enzyme activity.
 
Product No: KK-T**9
 
 

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