Recombinant Enterokinase high active

Description

Recombinant Enterokinase
High specificity and high purity
1. Product introduction
Enterokinase is a high-purity recombinant bovine enterokinase. The enzyme is purified by HPLC with high purity, high specificity and no other proteases. Enterokinase (EC 3.4.*1.9) is a specific protease that can cleave the carboxy-terminal site of lysine containing four aspartic acids in the front: Aspartic acid-Aspartic acid-Aspartic acid-Aspartic acid Aspartic acid-lysine. Enterokinase can remove the fusion protein located at the N-terminus of the protein to remove unwanted fusion tags.
 
2. Product advantages
high purity
   
High specificity
1) A specific protease cleaves the carboxy-terminal site of lysine containing four aspartic acids in front: aspartic acid-aspartic acid-aspartic acid-aspartic acid-lysine (DDDDK)
2) No other proteases, no non-specific cutting
Figure 1: SDS-PAGE detection result, a single main band of purified enterokinase
Figure 2: SDS-PAGE analysis of 1U of EK on *0ug substrate 0min, *0min, *0min, *0min, *0min, *0min, *0min
Figure 3: SDS-PAGE analysis of 1U, 2U, 5U, *0U EK on *0ug substrate for 4h and 1U EK on *0ug substrate for *6h
 
Enzyme digestion conditions:
According to the definition of enzyme activity, an example of recombinant EK digestion conditions: In *5mM Tris-HCl 8.0 system:
Fusion protein concentration 0.**1mg/ml (total protein *****0µg)
Reconstituted EK dosage **2U
Temperature *5℃
Overnight digestion, or complete digestion requires *2h**6h.
 
Common factors affecting enterokinase activity
At >**0mM imidazole, or >**0mMNaCl, or >5% glycerol, the digestion effect will be affected. You can refer to the following recommended methods for digestion:
1) In order to obtain an ideal digestion result, please dialyze the sample into *5mM Tris-HCl 8.0 buffer, and then perform digestion.
2) If it is inconvenient for dialysis, you can dilute the sample. The imidazole content is below **0mM, the NaCl concentration is below *0mM, and the glycerol concentration is below 5% for digestion, and the ratio of enzyme dosage to protein remains unchanged (ie 1U digestion *0μg protein) .
3) If the sample solution contains one or more of the above components and it is inconvenient to remove, at this time, increase the enzyme amount or extend the digestion time to achieve better digestion results.
 
3. Product use
Used to remove the fusion protein located at the N-terminus of the protein to remove unwanted fusion tags
 
4. Product features
Source Recombinant Escherichia coli
Theoretical MW: *5,**0DA
Activity 1U is defined as storing *0ug at *5℃, within *2h~*6h
The amount of enzyme required to cleave *5% of the fusion protein in *5mM Tris-HCl 8.0 buffer.
Storage Store at **0°C.
Stability It can be transported at room temperature and can be stored stably for one week at *5°C.
The enzyme solution can be repeatedly frozen and thawed several times without affecting the enzyme activity.
5. Product Information
Product name: Recombinant enterokinase
 Product number: KK-T**8
 Specific activity: ≥5u/μl