Recombinant Trypsin for biochemical high purity

Description

Product name: Recombinant Trypsin
CAS Number: ********7
Product Keywords: Recombinant Trypsin Trypsin Recombinant Trypsin Trypsin Recombinant Human Trypsin
Product categories: Chemicals, Biochemicals, Enzymes
Product attributes: hydrolases
Model: KK-T**3
Specification: mg, g
Trademark: Kekai
Packing: mg, g
product description:
Source: Recombinant Escherichia coli
Purification: HPLC
Product traits: white or almost white freeze-dried powder
Electrophoresis identification and Coomassie brilliant blue staining *2% SDS-PAGE: non-reducing SDS-PAGE protein electrophoresis, molecular weight *4kD, single main band
Protein content: *5%～*0%
Other enzyme content: no chymotrypsin, carboxypeptidase A and other pollution
Does not contain any protease inhibitors: no protease inhibitors such as PMSF, EDTA, etc.

1. Product introduction
Trypsin is a serine protease that cleaves peptide bonds at the C-terminus of lysine and arginine. Trypsin is widely used in various biotechnological processes. Such as: protein enzymatic hydrolysis to produce polypeptides; cell separation of various tissues; protein enzymatic hydrolysis and sequencing; recombinant insulin production and so on. Recombinant porcine trypsin is expressed and produced by recombinant E. coli, and its amino acid sequence is exactly the same as cationic trypsin derived from porcine pancreas. The specificity of restriction enzymes is the same. The molecular weight is *4kD, and the optimum pH is ***0. The activity is inhibited by serine protease inhibitors such as PMSF, and metal ion chelating agents such as EDTA inhibit enzymatic activity.

2. Product advantages
No animal origin: recombinant production, no foreign virus contamination, such as swine flu virus, porcine parvovirus, etc.
High purity: no other miscellaneous enzyme pollution: no other side reactions in the digestion reaction
High specific activity: no less than ***0 USP u/mg pro.
Stable: freeze-dried powder, easy to store and transport; mass production, stable product quality

3. Product use
Recombinant porcine trypsin has the same enzymatic properties as animal-derived porcine trypsin, and can replace porcine pancreas-derived trypsin in various biotechnological processes, such as: recombinant insulin production; cell culture, cell fermentation; protein enzymatic hydrolysis , Sequencing; cell separation of various tissues, etc.