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L-Carnitine test Kit

L-Carnitine test Kit

FOB Price

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Minimum Order

Place of Origin:

-

Price for Minimum Order:

-

Minimum Order Quantity:

1 Bag

Packaging Detail:

color box

Delivery Time:

7days

Supplying Ability:

500 Bag per Month

Payment Type:

T/T, Other

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REAGEN LLC

REAGEN LLC

China

Miembro Básico

Persona de contacto ANGELA

Shenzhen, Guangdong

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Description


Product Description
 
REAGEN™ L-CarnitineTest Kit is a simple convenient means of measuring L-Carnitine in biological samples such asmilk powder.
The unique features of the kit are:

  •  Low detection limit（2mg/**0g）
  • High reproducibility
  • A rapid robust enzyme-based assay.
 
Procedure Overview
 
REAGEN™ L-CarnitineTest Kit assay transfers an acetyl group from CoA to Carnitine and the free CoA formed is further processed with substrate to give absorbance (**5 nm).
 
Kit Contents, Storage and Shelf Life
 
Kit Contents Amount
Micro-wells *6wells
5 x Substrate 3ml,1bottle
L-Carnitine Standard  *2ug ,3bottle
Enzyme（CAT） *0ul,1bottle
 
 
REAGEN™L-CarnitineTest Kit Store the kit at4°C, protect from light.Read the entire protocol before performing the assay.
Required Materials Not Provided With the Kit
 
  • Microtiter plate reader (4*5 nm)
  • Vortex mixer
  • *-*0ul,  **-2*0ul and***-***0uL pipettes
  • Perchloric acid(AR)
  • KOH(AR)
  • pH meter
  • filter paper 
 
 
Reagent preparation
 
  • Perchloric acid(*3%): *3ml perchloric acid add *7ml water,mix.
  • KOH(4mol/L):*2.4g KOH add water to **0ml.
  • Carnitine Substrate(1x):get 0.5ml 5xCarnitine Substrate,add 2ml water,mix.
  • Enzyme（CAT）:get *0ul to centrifuge,Remove the supernatant,add **0ul water,mix.
  • Prepare the standards:add 0.5ml deionized  water to  Carnitine Standard bottle.Dilute*4ug/ml to*6,9.6,6.4,3.2,1.6 ug/ml
  µg/ mL *4ug/ml   µL Water  µL
STD1 1.6 *5 **5
STD2 3.2 *5 **5
STD3 6.4 *5 **5
STD4 9.6 *4 **2
STD5 *6 **0 **0
 
 
Sample Preparation
Milk powder
1) 2.5 gsample，add*5 mL *0℃water ,mix.。
2) Add5 mL *3% Perchloric acid，mix,stay *0mins.Add water to50ml，mix，filter to get the filtrate（must be clear solution）。
3) Get*0 mL filtrate，use 4 mol/L KOH to adjust pH *2.***3,*0ËšC water bath*0 min。Cool to room temperature,use *3% Perchloric acid to adjust pH to 7.0～7.5，
4) Add water to*0ml.mix,let stay for*2h in 4℃，getsupernatant to test.
Dilution factor: *0. (the filtrate must be clear)
 
 
Test protocol
 
1) Setfivestandard wells（1.6,3.2,6.4,9.6,*6）、sample well,
2) Add*00ul standard or sample to the Micro-well,
3) Add*0ul substrate,
4) Add *0ul water,At room temperature ,After 5 mins,**5nm detect OD1.
5) Add *0ul CAT,At room temperature ,After*0 mins,**5nm detect OD2
 
Concentration Calculations
A standard curve can be constructed by plotting the mean relative absorbance (%) obtained from each reference standard against its concentration in ng/mL on a  curve.
OD2-OD1 as the Vertical ordinate，concetration as the Horizontal coordinate.
 

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