Description

TESTPRINCIPLE
Porcine Chlamydia Ab ELISA Test Kit is an enzyme immunoassay designed to detect the presence of antibody to Chlamydia in the swine serum. A microtitration format has been configured by coating Chlamydia antigen in alternating columns on the plate. Upon incubation of the test sample in the coated well, antibody specific to Chlamydia forms a complex with the coated viral antigens.After washing away unbound material from the wells, an anti-porcine horseradish peroxidase conjugate is added that binds to any porcine IgG antibody attached in the wells. In the final step of the assay, unbound conjugate is washed away, and enzyme substrate and a chromogen, TMB,are added to the wells.
Stop the reaction by adding stop solution. Within *5 minutes read the absorbance at **0 nm using **0 nm as the reference wavelength if available.Enzyme immunoassays for the qualitative of IgG antibodies against Chlamydia in pig serum or plasma.