Description
The majority of glucoamylases are multidomain enzymes consisting of
a catalytic domain connected to a starch-binding domain by an
O-glycosylated linker region. Three-dimensional structures have
been determined of free and inhibitor complexed glucoamylases from
Aspergillus awamori var. X**0, Aspergillus niger, and
Saccharomycopsis fibuligera. The catalytic domain folds as a
twisted (alpha/alpha)(6)-barrel with a central funnel-shaped active
site, while the starch-binding domain folds as an antiparallel
beta-barrel and has two binding sites for starch or
beta-cyclodextrin. Description Glucoamylase Boli GA is made from
selected strains of Aspergillus niger using submerged fermentation,
extraction and refining techniques. Boli GA has a high conversion
rate of starchy substrates into fermentable sugars. It can
hydrolyze alpha-D*1,4 glucosidic bonds from the non-reducing end of
starch one after. It also hydrolyzes the alpha-D*1,6 glucoside
branch bonds of starch and cleaves the alpha*1,3 bonds, releasing
glucose. Boli GA has a robust glucoamylase activity. Boli GA is
widely applied to the production of alcohol, fuel ethanol, glucose,
antibiotics and brewing industries. It is used to saccharify
liquified mash from various sources including corn, milo, barley,
wheat, rice, potatoes and tapioca. This product is available both
in solid (powder) and liquid forms. Product Characteristics
Declared Enzyme: Glucoamylase Systematic Name: EC
3.2.1.3,1,*-alpha-D-glucan glucohydrolase Physical Form: Liquid
Solid powder Activity: **0,**0～**0,**0 U/ml **0,**0～**0,**0 U/g
Appearance: Light to dark brown Light yellow powder PH or Moisture:
3.0 to 5.0 ＜8% Specific gravity: 1.*0 to 1.*5 g/ml Effect of PH
For maximum activity, the optimal pH for Boli GA is in the range of
3.5 to 4.5. Effect of Temperature The optimal temperature range for
Boli GA is *8～*5°C(**7～**9°F).Temperatures above *5°C
(**9°F) can result in loss of activity. Benefits Faster
fermentation More output of ethanol Highly efficient in
starch/dextrin degradation Greatly improved performance in
pre-saccharification processes Less starch residues Dosage
Guidelines Boli GA is generally added at a level of 0.**2～0.**7%
w/w in ethanol processing (starch, dry solids basis, provided that
the enzyme activity is **0,**0 U/ml). The actual dosage depends on
the raw materials, temperature, reaction time, and pH of the
individual plant saccharification/fermentation process, and its
desired result.
