Description

Rat Endothelin 1( ET*1)ELISAKit

Please carefully read this instruction before using. This ELISA kit is based on the principle of double-antibody sandwich technique to detect Rat Endothelin 1( ET*1). Be used only for research purposes, not be used formedical diagnosis.

Intended Use
This kit is used to assay the Endothelin 1( ET*1)in the sample of Rat’s serum, blood plasma, and other related tissue Liquid.

Test principle
The kit uses a double-antibody sandwich enzyme-linked immunosorbent
assay (ELISA) to assay the level of Rat Endothelin 1（ ET*1）in samples. Add Endothelin 1（ ET*1） to monoclonal antibody Enzyme well which is pre-coated with Rat Endothelin 1（ ET*1）monoclonal antibody, incubation; then, add Endothelin 1 （ ET*1 ） antibodies labeled with biotin, and combined with Streptavidin-HRP to form immune complex; then carry out incubation and washing again to remove the uncombined enzyme. Then add Chromogen Solution A, B, the color of the liquid changes into the blue, And at the effect of acid, the color finally becomes yellow. The chroma of color and the concentration of the Rat Substance
Endothelin 1（ ET*1）of sample were positively correlated.

Materials supplied in the Test Kit

Materials required but not supplied

1. *7 °C incubator     
2. Standard Enzyme reader
3. Precision pipettes and Disposable pipette tips     
4. Distilled water
5. Disposable tubes for sample dilution          
6. Absorbent paper

Summary Procedure

Preparing reagents, samples and standards
Add prepared samples and standards, antibodies labeled with enzyme, reacting 60 minutes at *7 °
Plate washed five times, adding Chromogen solution A, B, reacting 10 minutes at *7 °C
Add stop solution
Measure the OD value within 10min
Package size:*6T per box.
Validity&Storage:Six months(**8°C)orTwelvemonths(**0°C)[see label on the outer box for the specific date].