Description
Source: Escherichia coli.
Molecular Weight: The recombinant Protein G, containing **6
amino acid residues which is amino acid ******4 of the
Streptococcus sp, has a predicted molecular mass of
approximately *1.6 kDa. But it migrates with an
apparent molecular mass of *2kDa in SDS-PAGE.
Quantity: 5mg/**0mg/1.0g
Purity: >*5% by SDS-PAGE analyses.
Specificity: 1. Binds with greater affinity to most mammalian
immunoglobulins than Protein A, including human
IgG3 and rat IgG2a.
2. Does not bind to human IgM, IgD and IgA.
Applications: Protein G binds to the constant region of many
species of immunoglobulin G. It can be used to detect,
quantify and purify IgG antibodies and antibody/antigen
complexes. PrimeGene’s Recombinant
Protein G contains only IgG binding domains. The
albumin-binding domain as well as cell wall and
cell membrane binding domains have been removed to ensure the
maximum specific IgG binding
capacity.
Physical Appearance: Sterile Filtered White lyophilized
(freeze-dried) powder.
Formulation: Lyophilized with no additive.
Endotoxin: Less than 0.1EU/µg of Protein G as determined by
LAL method.
Reconstitution: Dissolve in distilled water or saline.
Storage: 2 years at **0°C. After reconstitution, aliquot and
store at **0°C. Avoid repeated freeze/thaw cycles.
Usage: This material is offered by Fast Innovation Bioscience
for research, laboratory or further
evaluation purposes. NOT FOR HUMAN USE
