Description

Intended use
This immunoassay kit allows for the specific measurement of porcine ANG*2 concentrations in serum and plasma.

Introduction
Angiopoietin 2 and angiopoietin 1 have an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain. The coiled coil domains mediate ligand homo-oligomerization and the fibrinogen-like domains mediate ligand activity. Recombinant human angiopoietin 2 has a molecular mass of approximately *6 kDa in SDS-PAGE under reducing and non-reducing conditions. Angiopoietin 2 probably acts as a natural antagonist for Ang1 and Tie2 both in vivo and in vitro. In adult mice and humans, angiopoietin 2 is expressed at sites of vascular remodeling.

Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for ANG*2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANG*2 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for ANG*2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substporcinee solution is added to the wells and color develops in proportion to the amount of ANG*2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Related Products:
Human ANG2 ELISA Kit
Mouse ANG2 ELISA Kit
Porcine ANG2 ELISA Kit
Rat ANG2 ELISA Kit
Rabbit ANG*2 ELISA Kit