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What role does Tris buffer play in DNA extraction

China

DS-TRIS-08

( Negotiable ) Obtener el precio más reciente

Ezhou city,Hubei province,china

-

500 Gram

500g / bottle, 25kg / barrel

5 days

100 Ton per Month

T/T, L/C, PayPal, Other

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Especificaciones del producto

Other Names : tris(hydroxymethyl)aminoethane/Tromethamine/Aminobutanol MF : C4H11NO3 Purity (%) : 99 Classification : General Reagents Brand Name : Desheng CAS No. : - EINECS No. : -
Molecular Weight : 121.135 Appearance : White Crystal Powder Storage Conditions : Room Temperature, Light-proof And Moisture-proof

Descripción del producto

Tris, the full name of Tris, is a common biological buffer that can be used in the entire DNA extraction process. In the extraction process from multiple sources, DNA extraction is very sensitive to pH. In the process of cell lysis, removal of unwanted cell components and precipitation, Tris is required to maintain a stable pH. In addition, Tris buffer also plays a special role in cell lysis.

Lysis of cells is the first step in DNA extraction. This can be done with a buffer containing Tris and EDTA (ethylenediaminetetraacetic acid). EDTA binds divalent cations such as calcium and magnesium. Since these ions help maintain the integrity of the cell membrane, eliminating them with EDTA will destroy the stability of the cell membrane. Tris is the main buffer component; its main function is to maintain the pH of the buffer at a stable value (usually 8.0). In addition, Tris may interact with LPS (lipopolysaccharide) in the membrane, thereby further destabilizing the membrane.
 
Tris buffer protects DNA from changes in pH
When cells divide, their DNA and contents spill into the buffer. This cell content and fragmented RNA and protein have a great influence on the pH of the solution. Because DNA is sensitive to pH, it is important that Tris buffer keep the pH stable.
 
In the final stage of DNA extraction, the DNA itself is extracted from the solution. At this time, the DNA can be dissolved in the buffer. In order to extract from the solution, the DNA can be made insoluble by adding ethanol or isopropanol. After this operation is completed, the DNA will appear as a white thread-like substance in the solution. Although DNA can be separated from the remaining cellular components in this way, it is "unusable" when the DNA is insoluble. After separation, the alcohol is removed, and the DNA must be returned to the Tris buffer before it can be used.
 
In summary, Tris buffer has played many roles in DNA extraction, and it shows that Tris buffer plays an important role in it. This is also the reason why Tris buffer has become a hot commodity in the market, making the procurement work very difficult. Here we recommend our company, Hubei Xindesheng Material Co., Ltd..
 
As a manufacturer specializing in the production of biological buffers, Hubei Xindesheng Materials Co., Ltd. provides a large number of high-quality Tris and many other types of biological buffers. Our company can customize the product specifications and after-sales service according to customer needs, and we can provide professional testing services and packaging customization services to special customers. In any case, Desheng company must be the only one for you to buy biological buffers. select.
  What role does Tris buffer play in DNA extraction What role does Tris buffer play in DNA extraction What role does Tris buffer play in DNA extraction What role does Tris buffer play in DNA extraction

Contáctenos

Persona de contacto: jing zhao

Empresa:Hubei xindesheng Material Technology Co., Ltd

Dirección:Los Angeles, California, China,

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jing zhao < Hubei xindesheng Material Technology Co., Ltd >
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