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It is important to separate the cellular and liquid portions of a blood specimen as soon as possible when the test requires a sample of serum or plasma. This is because the cells interact with the serum/plasma, altering its chemical composition and affecting test results.
 
Centrifugal force is used to separate the components of blood  red blood cells, platelets and plasma from each other. The result is that the particles with different densities precipitate in layers.
 
For serum, allow the blood to clot sixty minutes and separate by centrifugation. For plasma and whole blood, completely fill the Vacutainer whenever possible to eliminate dilution from the anticoagulant or preservative and immediately mix the blood by gently and thoroughly inverting the tube five to ten times.
 
Draw *2 mL of whole blood for each 5 mL of serum or plasma needed. Collect in an appropriate collection tube. Centrifuge for at least *5 minutes at ********0 RPM. Pipette the serum or plasma into a clean plastic screw-cap vial and attach the label.