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India
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Column chromatography is the ideal method of chromatography for purification and separation of both solid and liquids. It is a solid or liquid technique in which the stationary phase is solid adsorbents like silica gel and activated alumina powder and mobile phase is a liquid. In solvent chromatography, the selection of solvent is based on both adsorbent and solvent. The principal of active compound separation is depends on the activity of adsorbents and polarity of the solvent. If the polarity of solvent is very low and activity of adsorbent is strong and high then result of separation of compound is good. On the other hand, if the polarity of solvent is very high and the activity of adsorbents is high then it gives poor results of compound separation. It means purification and isolation of compounds are not **0% pure.
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